可同时检测水中多种病毒的新技术
据新华社东京电 （记者蓝建中）日本一个研究小组日前宣布，他们开发出一种能够同时检测水中多种病原体病毒的技术。这种新技术最多可同时检测96种病毒。日本北海道大学与国立极地研究所的联合研究小组发表公报称，以往水质检验员在对上下水道的水质进行检测时，通常是通过调查容易检测的大肠杆菌来推测病原体病毒的量。此次开发的新技术能直接检测出多种主要病毒以及它们的含量。
这种新技术可检验的病毒包括会引发痢疾和呕吐的诺如病毒、轮状病毒以及会导致感冒的腺病毒等。今后，如果能在自来水厂和污水处理厂普及这一技术，将有助于进一步提高上下水道的安全性。
由于该技术使用了微小的检测容器，所以只需要很少的样本，8小时内就能得出结果。
Microfluidic Quantitative PCR for Simultaneous Quantification of Multiple Viruses in Environmental Water Samples
Satoshi Ishii1*, Gaku Kitamura1, Takahiro Segawa2,3, Ayano Kobayashi1, Takayuki Miura1, Daisuke Sano1 and Satoshi Okabe1
- Author Affiliations
1Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, Sapporo, Hokkaido, Japan
2National Institute of Polar Research, Tokyo, Japan
3Transdisciplinary Research Integration Center, Tokyo, Japan
ABSTRACT
To secure food and water safety, quantitative information of multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sapovirus, and hepatitis A and E viruses. Murine norovirus and mengovirus were also quantified in our MFQPCR system as a sample processing control and an internal amplification control, respectively. River water contaminated with effluents from a wastewater treatment plant in Sapporo, Japan, was collected and used to validate our MFQPCR system for multiple viruses. High-throughput quantitative information was obtained with a quantification limit of 2 copies/μl of cDNA/DNA. Using this MFQPCR system, we could simultaneously quantify multiple viral pathogens in environmental water samples. The viral quantities obtained using MFQPCR were similar to those determined by conventional qPCR. Thus, the MFQPCR system developed in this study can provide direct and quantitative information for viral pathogens, which is essential for risk assessments.
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