禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)
本帖转自 http://biosky.haotui.com/viewthread.php?tid=6402作者：pyau08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)
【作 者】：Erica Spackman Editor
【出 版 社】: Humana Press
【简 介】：
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The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
methods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
will be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
John M. Walker, the series editor, for his help in editing and working with me
through this project.
Erica Spackman
Athens, GA, September 2007
【出版日期】：2008
【目 录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
Erica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
Mary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
vii
9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents
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