[Journal Club] Mapping Viral Functional Domains for Genetic Diversity in Plants
Mapping Viral Functional Domains for Genetic Diversity in PlantsPita JS, Roossinck MJ. 2013. Mapping Viral Functional Domains for Genetic Diversity in Plants. J. Virol. 87:790–797.
粗略的中文摘要。。。
文章通过比较FNY 和LS两个株系的分离物寻找在植物里面控制遗传变化的因子。探究发现RNA1, RNA2控制遗传多样性的水平。进一步研究发现突变频率的控制因子在RNA1的前596氨基酸位置。2A蛋白中与2A/2B的重叠区域也与病毒遗传变化有关。在含有大多数固定突变的RNA3的3UTR发现一个多态性热点。沉默抑制因子2B基因通过依赖于寄主控制病毒种群的固定突变丰度
摘要：
Cucumber mosaic virus (CMV) comprises numerous isolates with various levels of in-host diversity. Subgroup-distinctive features of the Fny and LS strains provided us with a platform to genetically map the viral control elements for genetic variation in planta. We found that both RNAs 1 and 2 controlled levels of genetic diversity, and further fine mapping revealed that the control elements of mutation frequency reside within the first 596 amino acids (aa) of RNA 1. The 2a/2b overlapping region of the 2a protein also contributed to control of viral genetic variation. Furthermore, the 3′ nontranslated region (NTR) of RNA 3 constituted a hot spot of polymorphism, where the majority of fixed mutations found in the population were clustered. The 2b gene of CMV, a viral suppressor of gene silencing, controls the abundance of the fixed mutants in the viral population via a host-dependent mechanism
链接：
http://jvi.asm.org/content/87/2/790.full
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