[转移贴]-求 swine influenza H1N1 antibody IDEXX ELISA kit 说明书
liushuichong：IDEXX官网找不到，大家又的话，给发一份啊
hans：
ref:
The IDEXX Swine Influenza Virus H1N1 and H3N2 ELISAs were used according to the manufacturer's instructions.a,b The serum samples were diluted 1/40 in sample diluent, and 100 μl of positive and negative controls as well as diluted samples were added in duplicate to the wells. Thereafter, plates were incubated for 30 min. After removing the samples, plates were washed thoroughly, and 100 μl of an anti-porcine horseradish-labeled conjugate was added to all wells. Plates were again incubated for 30 min. Next, substrate (tetramethylbenzidine) was added after a washing step, and 15 min later the reaction was stopped with a sodium dodecyl sulfate solution. Next, optical densities (OD) were read at 650 nm in an ELISA plate reader. Results were expressed as a sample to positive (S/P) ratio by using the following formula: (mean sample OD – mean negative control OD)/(mean positive control OD – mean negative control OD). Samples with S/P ratios ≥0.4 were considered to be positive for SIV antibodies. For the H3N2 ELISA, S/P ratios <0.4 but ≥0.3 were classified as suspect. All other S/P ratios were classified as negative. All samples and controls were tested in duplicate, and the test was performed at room temperature. Arithmetic mean S/P ratios of positive samples were calculated.
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