4) 当序列检测预示疾病将发生，或序列拷贝数与疾病的严重程度有相关性，则序列与疾病的联系极可能构成因果关系。
| 我们一直在努力!4 d" c4 ~3 j5 ?) ?6 H
5) 从现有序列推断出的微生物特性应符合该生物类群的已知生物学特性。
病毒学论坛,自由学术论坛,病毒学,交流,病毒,免疫,virus,virology,immunology,cell culture. G9 w3 K# P0 _7 i# w! o' H" F: Y
6) 应在细胞水平探求患病组织与微生物的关系：用原位杂交来显示发生了组织病理变化的特定区域，以证明微生物的存在，或显示微生物应该存在的区域。 | 我们一直在努力!) x# r- \, K& T6 p
中国病毒学自由学术论坛, `1 _: Y- b2 c. _
7) 这些以序列分析为基础获得的上述证据应当是可重复获得的。
Here are Koch’s postulates for the 21st century as suggested by Fredricks and Relman:biosky.haotui.com* ^4 }6 \1 b9 G) d( w
1 A nucleic acid sequence belonging to a putative pathogen should be present in most cases of an infectious disease. Microbial nucleic acids should be found preferentially in those organs or gross anatomic sites known to be diseased, and not in those organs that lack pathology.
2 Fewer, or no, copy numbers of pathogen-associated nucleic acid sequences should occur in hosts or tissues without disease.
3 With resolution of disease, the copy number of pathogen-associated nucleic acid sequences should decrease or become undetectable. With clinical relapse, the opposite should occur.
4 When sequence detection predates disease, or sequence copy number correlates with severity of disease or pathology, the sequence-disease association is more likely to be a causal relationship.biosky.haotui.com' \. ?: K o; N: y6 B. x, O/ I
5 The nature of the microorganism inferred from the available sequence should be consistent with the known biological characteristics of that group of organisms.2 C+ F5 O# {' l- N/ M$ F
6 Tissue-sequence correlates should be sought at the cellular level: efforts should be made to demonstrate specific in situ hybridization of microbial sequence to areas of tissue pathology and to visible microorganisms or to areas where microorganisms are presumed to be located.
7 These sequence-based forms of evidence for microbial causation should be reproducible. | 我们一直在努力!+ L% } e B$ l6 @
Fredericks DN, & Relman DA (1996). Sequence-based identification of microbial pathogens: a reconsideration of Koch’s postulates. Clinical microbiology reviews, 9 (1), 18-33 PMID: 8665474中国病毒学自由学术论坛- }. U# Z0 [7 T) L2 O+ _+ X | 我们一直在努力!+ @. z8 i8 I# N
