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禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)
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2015-2-1 12:56
标题:
禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)
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http://biosky.haotui.com/viewthread.php?tid=6402
作者：
pyau
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08年新书 Avian Influenza Virus
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【书名版次】：
Avian Influenza Virus
（First edition）(Methods in Molecular Biology Series 436)
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【作 者】：Erica Spackman Editor
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【出 版 社】: Humana Press
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【简 介】：
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08新书,大家共享
附件PDF下载！[attach]70[/attach]
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The aim of this book is to create a resource that includes both basic methods that
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are currently used and well established as well as some of the most exciting new
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methods for studying the virus itself, and to include methods that focus on work
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with avian hosts, an area that has been greatly lacking. In that context, this volume
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will be of interest to both diagnosticians and researchers, but it does assume a basic
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knowledge of virology and molecular biology.
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I would like to thank all the authors for their contributions. I am also thankful to
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John M. Walker, the series editor, for his help in editing and working with me
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through this project.
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Erica Spackman
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Athens, GA, September 2007
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【出版日期】：2008
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【目 录】：
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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
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Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
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1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
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Erica Spackman
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2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
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Mary Lea Killian
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3 Avian Infl uenza Virus RNA Extraction from Tissue
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and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
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Erica Spackman and David L. Suarez
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4 Type A Infl uenza Virus Detection and Quantitation
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by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
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Erica Spackman and David L. Suarez
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5 Detection and Identifi cation of the H5 Hemagglutinin
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Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
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Erica Spackman and David L. Suarez
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6 Avian Infl uenza Virus Isolation and Propagation
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in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
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Peter R. Woolcock
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7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
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Mary Lea Killian
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8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
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Subtype Identifi cation and the Detection and Quantitation
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of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
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Janice C. Pedersen
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9 Neuraminidase-Inhibition Assay for the Identifi cation
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of Infl uenza A Virus Neuraminidase Subtype or
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Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
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Janice C. Pedersen
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10 Immunohistochemical Staining for the Detection of the
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Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
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Mary J. Pantin-Jackwood
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11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
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Justin D. Brown and David E. Stallknecht
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12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
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Chang-Won Lee and David L. Suarez
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13 Evaluating the Cell-Mediated Immune Response of Avian
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Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
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Darrell R. Kapczynski
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14 Measurement of Avian Cytokines with Real-Time RT-PCR
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Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
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Darrell R. Kapczynski and Michael H. Kogut
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Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
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viii Contents
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