课题研究得到亚特兰大疾病控制和预防中心全球疾病监测项目资助。 作者: rojjer 时间: 2015-8-8 15:04
ipsvirus
A distinct lineage of influenza A virus from bats
Suxiang Tonga,1, Yan Lia, Pierre Rivaillerb, Christina Conrardya, Danilo A. Alvarez Castilloc, Li-Mei Chenb, Sergio Recuencod, James A. Ellisond, Charles T. Davisb, Ian A. Yorkb, Amy S. Turmelled, David Moranc, Shannon Rogersa, Mang Shia, Ying Taoa, Michael R. Weile, Kevin Tangf, Lori A. Rowef, Scott Sammonsf, Xiyan Xub, Michael Fracef, Kim A. Lindbladeg, Nancy J. Coxb, Larry J. Andersona, Charles E. Rupprechtd,1, and Ruben O. Donisb,1
Influenza A virus reservoirs in animals have provided novel genetic elements leading to the emergence of global pandemics in humans. Most influenza A viruses circulate in waterfowl, but those that infect mammalian hosts are thought to pose the greatest risk for zoonotic spread to humans and the generation of pandemic or panzootic viruses. We have identified an influenza A virus from little yellow-shouldered bats captured at two locations in Guatemala. It is significantly divergent from known influenza A viruses. The HA of the bat virus was estimated to have diverged at roughly the same time as the known subtypes of HA and was designated as H17. The neuraminidase (NA) gene is highly divergent from all known influenza NAs, and the internal genes from the bat virus diverged from those of known influenza A viruses before the estimated divergence of the known influenza A internal gene lineages. Attempts to propagate this virus in cell cultures and chicken embryos were unsuccessful, suggesting distinct requirements compared with known influenza viruses. Despite its divergence from known influenza A viruses, the bat virus is compatible for genetic exchange with human influenza viruses in human cells, suggesting the potential capability for reassortment and contributions to new pandemic or panzootic influenza A viruses.
Li Q, Sun X, Li Z, Liu Y, Vavricka CJ, Qi J, Gao GF.
The recent discovery of the unique genome of influenza virus H17N10 in bats raises considerable doubt about the origin and evolution of influenza A viruses. It also identifies a neuraminidase (NA)-like protein, N10, that is highly divergent from the nine other well-established serotypes of influenza A NA (N1-N9). The structural elucidation and functional characterization of influenza NAs have illustrated the complexity of NA structures, thus raising a key question as to whether N10 has a special structure and function. Here the crystal structure of N10, derived from influenza virus A/little yellow-shouldered bat/Guatemala/153/2009 (H17N10), was solved at a resolution of 2.20 ?. Overall, the structure of N10 was found to be similar to that of the other known influenza NA structures. In vitro enzymatic assays demonstrated that N10 lacks canonical NA activity. A detailed structural analysis revealed dramatic alterations of the conserved active site residues that are unfavorable for the binding and cleavage of terminally linked sialic acid receptors. Furthermore, an unusual 150-loop (residues 147-152) was observed to participate in the intermolecular polar interactions between adjacent N10 molecules of the N10 tetramer. Our study of influenza N10 provides insight into the structure and function of the sialidase superfamily and sheds light on the molecular mechanism of bat influenza virus infection.
Proc Natl Acad Sci U S A. 2012 Oct 25. [Epub ahead of print]
The neuraminidase of bat influenza viruses is not a neuraminidase.
García-Sastre A.
Source
Department of Microbiology, Department of Medicine, Division of Infectious Diseases, and Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, New York, NY 10029. 作者: rojjer 时间: 2015-8-8 15:07
deepblue
也有人持异议，包括一些美国院士。主要担心病毒没有成功拯救出来，缺少最关键的一条定论数据。再继续观察吧作者: rojjer 时间: 2015-8-8 15:07
^_^
请教一个问题：除了唾液酸受体外，甲型流感病毒有没有其它的受体？
