Prevalence of a virus similar to human hepatitis B virus in swine
Author: Li, W.; She, R.; Liu, L. (...)
Source: Virol J, 2010, 7(1): 60 http://www.virologyj.com/content/7/1/60
Background
The objective of this study is to established evidence of the existence of a novel member of the hepadnavirus family endemic in swine. Temporarily this virus was designated as swine hepatitis B virus (SHBV). This SHBV can be detected by using human hepatitis B virus diagnostic kits including ELISA, immunohistochemical staining, and transmission electron microscopy (TEM). Also seroprevalence of pig farms in Beijing, China, and pathological features of SHBV infection was determined.
Results
Screened result shows that overall prevalence of HBsAg was 24.8%, closed to that of anti-HBsAg, whereas HBeAg and anti-HBe were barely detectable. The distribution of HBsAg and HBcAg was examined by immunohistochemistry of liver samples. Typical hepatitis pathological change, such as spotty parenchymal cell degeneration, necrosis of hepatocytes and proliferation of fibrous connective tissue were observed during histopathological analysis. Analysis of HBsAg-positive serum with TEM revealed two morphologic forms, 20nm and 40nm sized particles, similar to small spherical and Danes particles of HBV. Observation of the ultrastructure of the liver also found HBV-like particles in the nucleus of hepatocytes.
Conclusion
Our research result implies that SHBV could be a causative agent of swine. The discovery of SHBV will unveil novel evolutionary aspects of hepatitis and provides new information for further hepadnavirus research. 作者: cao1976 时间: 2015-8-23 22:16
dudiaojiangxue 发表于 2010-4-1 09:00 ：
这就好玩儿了，乙肝研究正缺动物模型呢。现有的DHBV鸭肝模型由于和HBV差别比较大，一直不太理想。所以很多研究组一直在做老鼠的动物模型，但老鼠不能直接被HBV感染的，所以还得做嵌合体，总之麻烦大了。猪动物模型有可能会缓解乙肝动物模型缺乏这一问题。