Influenza virus genome C4 promoter/origin attenuates its transcription and replication activity by the low polymerase recognition activity
Hongbing Jiang1, a, Shijian Zhang1, a, Qiang Wanga, Jinlan Wanga, Liqing Genga and Tetsuya Toyoda, a,
A natural variation is observed at position 4 of the 3′-end of influenza A virus genomes, where U (U4) or C (C4) is present. The replicon activity of C4 was 28% of U4. We compared the transcription and replication activity of U4 [v84(U4)], C4 [v84(C4)] and the complimentary RNA (c84) using the purified influenza virus RNA polymerase in vitro. ApG-primed replication activities of v84(C4) and c84 were 23.8% and 7.8% of v84(U4). Globin mRNA-primed transcription activities of v84(C4) and c84 were 36.9% and 6.81% of v84(U4). De novo replication activities of v84(C4) and c84 were 21.3 and 10.2% of v84(U4). This difference came from their polymerase binding activity. When all the eight genome segments of WSN strain were changed to U4, the virus titer was 760 times higher than the wild type. However, its pathogenicity in mice was lower than the wild type. 作者: rojjer 时间: 2015-10-10 12:19
tatamige：
又是一篇反向遗传技术的经典应用啊 顶一个