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演讲题目：The Infectious Cycle, H2 s+ y$ O0 D/ T8 h" {( d
演讲者：Prof. Vincent Racaniello, Ph.D.3 @! X2 @7 J8 `& E5 ~3 q+ H* m } n
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演讲者单位：哥伦比亚大学
' R6 g: u) k; k9 H+ i内容简介：主要讲解了下病毒感染细胞的过程，从吸附进入到病毒基因组和结构蛋白的合成，组装，到最后病毒粒子的释放。不同种类RNA病毒的复制周期。详细讲解了正链RNA病毒，负链RNA病毒，双链RNA病毒的复制机理。适合中级进阶者学习和练习英文之用。
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总时长65分钟，1080高清，总计约210M，强烈建议在wifi环境下打开观看。即便是土豪也请低调下吧。因为后续我们还将继续上传高清公开课视频哦) G3 U( q; V0 ^, D3 V" `, n+ t
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- J/ [, B4 [( d& D4 n精彩内容展示：
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! w, L9 q- j# F) x1 L# R# g首先讲解The Infectious Cycle的概要图，如下3 i1 d5 L7 I/ m5 s2 V' ?
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- Z( k4 b# O. w. l4 u$ \" p随后是阐述了关于病毒感染的细胞的4个重要概念，即：
0 b9 |4 K2 U6 [- m5 dA susceptible cell has a functional receptor for a given virus - the cell may or may not be able to support viral replication
9 D) @5 A5 r5 t# F, _. }* O2 j. ~A resistant cell has no receptor - it may or may not be competent to support viral replication& K; e' ?: Y" j9 U2 _2 |$ }
A permissive cell has the capacity to replicate virus - it may or may not be susceptible! m2 y( |% U0 a
A susceptible AND permissive cell is the only cell that can take up a virus particle and replicate it( H6 \: G$ A& n. i* D
* p* E$ N) W4 @7 E) n 病毒的常见宿主' g `; j* m3 P* e
# P4 z: s) S5 M" WAnimal viruses at first could not be routinely propagated in cultured cells，Most viruses were grown in laboratory animals，其中鸡胚对多种病毒敏感
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3 D7 t& R2 j/ J% n, ~8 Y/ C+ Q 没有细胞培养，就没有病毒感染过程的发现，该发现获得了1954年诺贝尔奖。3 _6 X' A# `2 C. j# p( |
9 P4 T1 i4 D- W* i% H1952年建立空斑技术，此项发明获得了1975年诺贝尔奖。
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Multiplicity of infection (MOI)的定义, V+ t2 V% _. H
Number of infectious particles ADDED per cell
; d. Y8 d+ X& x SNot the number of infectious particles each cell receives2 Y* F# S% V5 Z1 c8 ]2 |
Add 107 virus particles to 106 cells - MOI of 10 - each cell does NOT receive 10 virions
, ~/ t$ M# ^3 x6 X R' [Infection depends on the random collision of virions and cells' N1 u4 q9 }6 ]* q
When susceptible cells are mixed with virus, some cells are uninfected, some receive one, two, three or more particles& X" o/ }" S. Y/ H! B
The distribution of virus particles per cell is best described by the Poisson distribution
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病毒粒子常见的计数方式
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附 Vincent R. Racaniello教授简介
4 L* w4 T2 V, y4 j6 K! Z Prof.Vincent R. Racaniello (born January 2, 1953 in Paterson, New Jersey) is a Higgins Professor in the Department of Microbiology and Immunology at Columbia University’s College of Physicians and Surgeons. He is one of four virologists who has recently authored Principles of Virology, a textbook used by many teaching virology to undergraduate, medical and graduate students.
& S+ {) X3 Z" p* ?5 L* G( a& u As an esteemed member of the scientific community, Racaniello has received several awards including Irma T. Hirschl, Searle Scholars, Eli Lilly and NIH Merit. He has also been a Harvey Society Lecturer at Rockefeller University, the Hilleman Lecturer at the University of Chicago, and University Lecturer at Columbia University. Racaniello has served on the editorial boards of scientific journals, including the Journal of Virology, and is a community editor for the open access journal PLOS Pathogens. He also served as the 2015 president of the American Society for Virology. |
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发表于 2017-3-16 23:19:19
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