禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

wwwkkk83

本帖转自 http://biosky.haotui.com/viewthread.php?tid=6402  作者：pyau

08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
. ?" C! l  n# ?- T' @+ a【出 版 社】: Humana Press
【简    介】：
   08新书,大家共享    附件PDF下载！
+ }( J: b7 d1 A5 H+ F0 Q
3 R8 ]) r, w+ I/ r   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
; V7 c, V! b7 e4 bmethods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
+ @3 g( E8 v8 e2 J/ |; B" Xwill be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
6 R" @5 T( M8 mI would like to thank all the authors for their contributions. I am also thankful to
6 E+ g; p. K- @7 u% kJohn M. Walker, the series editor, for his help in editing and working with me
through this project.
1 |8 J  B, T9 R& ^, dErica Spackman
Athens, GA, September 2007
/ ^$ v( E; t7 G, {9 M2 q1 N
* b; G/ V( A* G9 ?( z' ]5 c【出版日期】：2008
4 ~8 x1 _  e& W1 l【目    录】：
, `0 [) _( s! r+ x" FPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
9 ?0 B) i3 u( r# p% z- aContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
- R" K0 X: s* i; yErica Spackman
' K; n. J6 y6 \) T5 A0 B2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
Mary Lea Killian
- b+ K/ r3 k; F3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
: X: L7 U$ w0 g; m, S4 Type A Infl uenza Virus Detection and Quantitation
2 Q. W! Q7 @4 _, I6 Uby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
/ Z/ ?: X, e7 O9 W0 `% [Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8 ^7 Q  ~& o% K9 F) K1 HErica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
$ n! ?+ w/ ?# J9 w( a3 P% E% a7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
9 N& U3 ^7 r6 W8 j$ }0 ?& kMary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
- I' ^0 @1 ], K5 eof Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
vii
: ~, d9 N. L& f9 B9 P9 Neuraminidase-Inhibition Assay for the Identifi cation
, o# H- }* {5 M# K& ]of Infl uenza A Virus Neuraminidase Subtype or
. U# E; s$ ]# R4 T' WNeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
" ?1 h; V  T. s! y" |- SJanice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
6 f6 R0 _9 g2 ?9 T3 C$ |0 BAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
1 j, q5 h# R" p. F' \0 |Mary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
3 d% q$ ~* S8 X! R+ Q7 X13 Evaluating the Cell-Mediated Immune Response of Avian
( s+ y) B, A1 k" f$ U7 |: Q- n# OSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
' O1 z0 u7 G9 O* j/ W! m6 }Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
% U8 N; Z. q4 pDarrell R. Kapczynski and Michael H. Kogut
/ K' A8 M" l0 Z  t+ G( iIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents