禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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! W2 V# l: \! @( Y* {2 s* w08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
# m) v& P$ Y1 N【简    介】：
   08新书,大家共享    附件PDF下载！
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$ n. y1 j3 I) T   The aim of this book is to create a resource that includes both basic methods that
  o/ P8 @# v. d4 z2 U. H2 |6 d; T# nare currently used and well established as well as some of the most exciting new
8 B6 w2 `# ]" Q; B0 f' S; k' Q& _methods for studying the virus itself, and to include methods that focus on work
% Z7 Z3 H. q0 W, |4 d) Z5 lwith avian hosts, an area that has been greatly lacking. In that context, this volume
: U  ?% s3 o% `' Z5 Jwill be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
. [: {3 r2 h5 g  A/ h0 O2 q; b) MI would like to thank all the authors for their contributions. I am also thankful to
8 ?2 |  J6 s  Q; f' |John M. Walker, the series editor, for his help in editing and working with me
% b; B6 N! L/ ithrough this project.
Erica Spackman
5 T% _$ }  ?+ @( b- b9 G5 C8 OAthens, GA, September 2007
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/ ~: l" g# [7 O( S! G2 a& b5 v【出版日期】：2008
* L8 J1 Z; Y) I! Y4 a【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
9 h1 W8 M/ q! X8 H6 T1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
Erica Spackman
6 `) F. \; p/ V. c) W2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
( e3 w1 T' {( U3 a: D* p/ {Mary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
0 g# ~( o; u; i6 ?0 dErica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
- r( ^! I4 x8 A2 pby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
% j; v# i) T) `5 v" X5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
* M: A) c. V; d7 {; {5 bErica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
$ @& n. }$ s; k7 s% I1 f9 ein Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
: ?, t8 F1 p3 r7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
6 @( X5 G) j7 L7 z6 @Subtype Identifi cation and the Detection and Quantitation
$ \0 T& n; Y) k+ ^of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
, ~, X/ Q4 B; [, c/ gJanice C. Pedersen
2 V% I! m4 o$ |4 Q8 Zvii
1 @( j0 S6 D0 o0 j, H9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
9 k/ J7 O; L: `; M* yNeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
6 Q  H' b2 V, r2 V* T6 g* U10 Immunohistochemical Staining for the Detection of the
& V% k! u) G7 |& |Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
& E2 g" e9 d& T3 m7 o* I" WMary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
1 ]3 G- E5 z; J5 H/ U1 R, fJustin D. Brown and David E. Stallknecht
- |3 P" v5 o. p4 l4 J12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
( `/ z. i( `1 s) _, G: pChang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
5 H' ?2 c/ ]2 @' ]" R$ a4 CSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
! O! E6 u) g+ X% rIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents
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