禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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* F; j& V3 L, t* y1 b% y08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
, L2 t0 J' w5 q【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
! z3 {! m0 ]+ f【简    介】：
   08新书,大家共享    附件PDF下载！
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" z1 O' C- R  I) ~5 ]" a* A2 a( g% W   The aim of this book is to create a resource that includes both basic methods that
& V* q4 u. g5 [/ D+ H: n+ T; yare currently used and well established as well as some of the most exciting new
- N6 M  G) L. r8 cmethods for studying the virus itself, and to include methods that focus on work
+ _- z: ~0 Y0 |with avian hosts, an area that has been greatly lacking. In that context, this volume
will be of interest to both diagnosticians and researchers, but it does assume a basic
$ |$ c0 |" d; q2 Dknowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
4 `, p, M% O8 G# p% Q6 j) MJohn M. Walker, the series editor, for his help in editing and working with me
through this project.
Erica Spackman
# u# b1 I: X8 z8 o4 A4 ~Athens, GA, September 2007

【出版日期】：2008
【目    录】：
2 T2 F! c7 w. _& K7 n5 d( j0 R) xPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
3 v- m8 f1 M& B. ?/ OErica Spackman
, j' ]* N& G5 F2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
" c7 S2 _2 R# b( j- |! d  Y% eMary Lea Killian
4 u. Y9 h3 v0 y( o# _. g6 t3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4 P2 G) z; ^, mErica Spackman and David L. Suarez
# b# x# i) R$ h' }. _4 Type A Infl uenza Virus Detection and Quantitation
; }! D' j' M2 m8 p; v* u+ F3 Iby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
' u. t. A4 Z5 d+ i9 EErica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
$ k5 F) O2 ?  Z: \  C7 PSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
4 [. s0 Z9 F* m9 Y9 C6 Avian Infl uenza Virus Isolation and Propagation
! p  s2 K* `) t. v0 E  w, kin Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Peter R. Woolcock
( w  B" U% X$ a* m7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
' U+ b2 f5 V- y8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
& N- v! t9 _0 d4 l! [of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
# v% M- o& r0 V7 z2 [; k7 uJanice C. Pedersen
  H: P( p3 A$ w, dvii
: F# t$ O. T, d( [& C9 Neuraminidase-Inhibition Assay for the Identifi cation
7 B* |" r) [) O1 zof Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
/ j7 s2 F: [: {: N: q- A# b10 Immunohistochemical Staining for the Detection of the
- L3 ~3 V$ m6 {6 X2 h1 U3 dAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
2 H: ]% |. L* i% f11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
: E- |% K3 W* W& Y" ]) uJustin D. Brown and David E. Stallknecht
! h( C& c3 J) w5 u12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
, W6 x$ r5 B2 A' c14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents
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