[转移帖]谷丙转氨酶(ALT)催化活性表位

hantavirus

原帖由lucky发表于 2010-6-1 14:42 |

大家好!受朋友之托,到坛子里问几个问题:
1.谷丙转氨酶(ALT)的氨基酸序列是什么?
2.ALT的催化活性表位是线性的还是构象依赖性的?
3.ALT的催化活性位点的氨基酸序列是什么?
由于本人不是从事肝炎病毒研究,对于ALT的生化活性了解甚少,希望能够得到大家的帮助,在此代朋友谢谢大家!

hantavirus

bigben发表于 2010-6-3 00:27 ：


This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

1
谷丙转氨酶蛋白序列链接
http://www.ncbi.nlm.nih.gov/protein/4885351
http://www.uniprot.org/uniprot/P24298
核酸序列链接
http://www.ncbi.nlm.nih.gov/nuccore/NM_005309.2
基因介绍链接：
http://www.ncbi.nlm.nih.gov/gene/2875#geneReference sequences

谷丙转氨酶蛋白序列：
        1 masstgdrsq avrhglrakv ltldgmnprv rrveyavrgp ivqraleleq elrqgvkkpf
       61 teviranigd aqamgqrpit flrqvlalcv npdllsspnf pddakkraer ilqacgghsl
      121 gaysvssgiq liredvaryi errdggipad pnnvflstga sdaivtvlkl lvageghtrt
      181 gvlipipqyp lysatlaelg avqvdyylde erawaldvae lhralgqard hcrpralcvi
      241 npgnptgqvq trecieavir fafeerlfll adevyqdnvy aagsqfhsfk kvlmemgppy
      301 agqqelasfh stskgymgec gfrggyvevv nmdaavqqqm lklmsvrlcp pvpgqalldl
      361 vvsppaptdp sfaqfqaekq avlaelaaka klteqvfnea pgiscnpvqg amysfprvql
      421 ppraveraqe lglapdmffc lrlleetgic vvpgsgfgqr egtyhfrmti lppleklrll
      481 leklsrfhak ftleys

2，3
我不太了解结构方面的内容，也不知道你问线性表位和构象表位的原因，抗体吗？
我查了一下，谷丙转氨酶催化结构属于一个AAT_like的结构，底下链接有结构、催化氨基、文献信息
domain分析
http://www.ncbi.nlm.nih.gov/Stru ... EQUENCE=NP_005300.1

AAT_like结构信息
http://www.ncbi.nlm.nih.gov/Stru ... ;uid=99734#seqalign