Contents
Preface ..............................................................................................................v
Contributors .................................................................................................. xiii
PART I. DNA VACCINE DESIGN
1 DNA Vaccine Design
Janet L. Brandsma ................................................................................. 3
2 Design of Plasmid DNA Constructs for Vaccines
Donna L. Montgomery and Kristala Jones Prather ............................. 11
3 Vaccination With Messenger RNA
Steve Pascolo ...................................................................................... 23
4 A Stress Protein-Facilitated Antigen Expression System
for Plasmid DNA Vaccines
Petra Riedl, Nicolas Fissolo, Jörg Reimann,
and Reinhold Schirmbeck ............................................................... 41
5 In Vitro Assay of Immunostimulatory Activities
of Plasmid Vectors
Weiwen Jiang, Charles F. Reich, and David S. Pisetsky ..................... 55
PART II. DNA VACCINE DELIVERY SYSTEMS
6 Delivery of DNA Vaccines Using Electroporation
Shawn Babiuk, Sylvia van Drunen Littel-van den Hurk,
and Lorne A. Babiuk ....................................................................... 73
7 Needle-Free Injection of DNA Vaccines: A Brief Overview
and Methodology
Kanakatte Raviprakash and Kevin R. Porter ....................................... 83
8 Needle-Free Delivery of Veterinary DNA Vaccines
Sylvia van Drunen Littel-van den Hurk, Shawn Babiuk,
and Lorne A. Babiuk ....................................................................... 91
9 Surface-Modified Biodegradable Microspheres
for DNA Vaccine Delivery
Mark E. Keegan and W. Mark Saltzman ........................................... 107
10 A Dendrimer-Like DNA-Based Vector for DNA Delivery:
A Viral and Nonviral Hybrid Approach
Dan Luo, Yougen Li, Soong Ho Um, and Yen Cu ............................. 115
11 Identification of Compartments Involved in Mammalian
Subcellular Trafficking Pathways by Indirect
Immunofluorescence
Anne Doody and David Putnam ....................................................... 127
PART III. DNA VACCINE ADJUVANTS AND ACTIVITY ENHANCEMENT
12 Adjuvant Properties of CpG Oligonucleotides in Primates
Daniela Verthelyi .............................................................................. 139
13 Complexes of DNA Vaccines With Cationic, Antigenic Peptides
Are Potent, Polyvalent CD8+ T-Cell-Stimulating Immunogens
Petra Riedl, Jörg Reimann, and Reinhold Schirmbeck ..................... 159
14 Prime-Boost Strategies in DNA Vaccines
C. Jane Dale, Scott Thomson, Robert De Rose, Charani
Ranasinghe, C. Jill Medveczky, Joko Pamungkas,
David B. Boyle, Ian A. Ramshaw, and Stephen J. Kent ................ 171
15 Modifying Professional Antigen-Presenting Cells to Enhance
DNA Vaccine Potency
Chien-Fu Hung, Mu Yang, and T. C. Wu .......................................... 199
16 Replicase-Based DNA Vaccines for Allergy Treatment
Sandra Scheiblhofer, Richard Weiss, Maximilian Gabler,
Wolfgang W. Leitner, and Josef Thalhamer ................................. 221
PART IV. DNA VACCINE APPLICATIONS
17 Immunological Responses of Neonates and Infants
to DNA Vaccines
Martha Sedegah and Stephen L. Hoffman ........................................ 239
18 DNA Vaccines for Allergy Treatment
Richard Weiss, Sandra Scheiblhofer,
and Josef Thalhamer ..................................................................... 253
19 Protection From Autoimmunity by DNA Vaccination
Against T-Cell Receptor
Thorsten Buch and Ari Waisman ...................................................... 269
20 The Use of Bone Marrow-Chimeric Mice in Elucidating
Immune Mechanisms
Akiko Iwasaki .................................................................................... 281
PART V. DNA VACCINE PRODUCTION, PURIFICATION, AND QUALITY
21 A Simple Method for the Production of Plasmid DNA
in Bioreactors
Kristin Listner, Laura Kizer Bentley, and Michel Chartrain ............. 295
22 Practical Methods for Supercoiled pDNA Production
John Ballantyne ................................................................................. 311
23 Production of Plasmid DNA in Industrial Quantities According
to cGMP Guidelines
Joachim Schorr, Peter Moritz, Astrid Breul,
and Martin Scheef ........................................................................ 339
24 Large-Scale, Nonchromatographic Purification of Plasmid DNA
Jason C. Murphy, Michael A. Winters,
and Sangeetha L. Sagar ................................................................. 351
25 Assuring the Quality, Safety, and Efficacy of DNA Vaccines
James S. Robertson and Elwyn Griffiths ........................................... 363
Index ................................................................................................. 375 作者: zhaox 时间: 2015-12-1 23:31
一共7个分卷压缩包。