标题: Virologica Sinica 精彩论文抢先看！ [打印本页] 作者: lishaojie 时间: 2015-12-7 17:01 标题: Virologica Sinica 精彩论文抢先看！ Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF) " z% u; G# C5 B Q' U0 ]Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi* 9 f4 x O' m& v+ y % L; ~. Z0 A* ?, E0 h3 }7 \Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats. / `1 B! \9 \5 N* ATherefore, bats may have evolved effective mechanisms to control viral replication. However, little- }/ h1 T7 c" z. L T+ {
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a6 h2 R% X a0 ^
key role in controlling viral infections. In this study, we report the cloning, expression, and 2 N" B: z6 ?7 [5 ^1 Q* }. M/ {8 l, lbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We ) Y6 E9 P9 o8 j( Bdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from l* G+ e8 a+ ]; @6 U9 Q7 ]+ p
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant# M1 S1 g! E6 w
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat+ |: ?: e; U0 [6 f! n1 N, |8 `8 W
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral% y. B% u# }$ k' v, O# V
activity in microbats, which has important implications for virus interactions with these hosts. 1 U( C* x9 Z% ~7 R# g; `! S( [, b. |9 d3 @& G2 W% u" G Keywords: bat; interferon; IFN-stimulated genes; antiviral activity' U3 T* Q: K8 s5 g$ s* R
- s8 B. g, T, u2 [* U" m3 T . {0 |: b( A* X7 }& h* j, z5 }中文信息： 2 f6 m, A- d+ M4 [# e 9 z0 E3 ]2 |& S. X大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测 : O9 j4 I: U% y9 H- {* P! n/ E( v& M 5 v, c+ G. \, O R, b6 F' i , Z( w; V q) G( Z7 d5 H梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽* 5 F: r6 e* v R/ G- o. K& x) h; ? + d3 j' I" J3 P0 e' g6 [' M: B9 f1 i" z 摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。7 w9 q4 Q, s# o0 h2 @* J
: q$ j# z3 K ~ d$ k 9 U" R' L* a' R3 \1 I1 x+ Y t关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR( m. d B. H" X$ r: j. j/ M9 k. U. T
# C8 c( R4 W" d4 G1 { 作者: rojjer 时间: 2015-12-7 22:28
不错！