禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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; r! _; ]3 o. S" I08年新书 Avian Influenza Virus
4 D: [' b; A& h: [# o* t% l  J【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
【简    介】：
   08新书,大家共享    附件PDF下载！

   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
% ]+ G2 O" I. }& S2 n' rmethods for studying the virus itself, and to include methods that focus on work
$ c  L- u+ x) S6 I- x, Vwith avian hosts, an area that has been greatly lacking. In that context, this volume
& i! \9 T3 I- Z* Mwill be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
4 p( g! w1 U' ]) e/ n6 d/ j* _John M. Walker, the series editor, for his help in editing and working with me
through this project.
Erica Spackman
Athens, GA, September 2007

7 g3 j! {' h" s+ `% L. C【出版日期】：2008
; g2 ^' z1 C! D1 F9 B: j+ D【目    录】：
0 U) n, y1 z- t. w' hPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
: K+ _% S  Q2 w  MContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
& W" }( U3 s* l5 s3 i9 i" s- Q$ y1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
: p% c5 Y3 r) p# Z6 |Erica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
$ Y: y- E9 P' VMary Lea Killian
5 x' U; s" o5 u3 Avian Infl uenza Virus RNA Extraction from Tissue
0 w- P! r3 _% b7 rand Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3 P# w5 |# p% U2 [/ JErica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
7 q0 V0 _! k# W7 Q8 c4 Tby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
& @- Q8 Q7 _4 [0 \1 s5 \# G" G' A8 VErica Spackman and David L. Suarez
. |6 _# c& e! O( S4 v0 M9 f6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
, j# Z8 B4 b  I; hPeter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
1 x2 u# z$ z; t# [: K' G* R: MMary Lea Killian
( q. {. L! M& X0 N9 ~6 J5 t- `8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
6 |2 h: o! L# Q) k3 ~Subtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
. i+ `4 o$ }; Q, a( wJanice C. Pedersen
9 o3 u7 o! a1 p* k9 i$ zvii
9 Neuraminidase-Inhibition Assay for the Identifi cation
/ L3 k- d- J7 I: X. I3 V+ G+ Cof Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
+ D5 A; b0 Q3 i# q! z3 b# ?Janice C. Pedersen
6 \  ]  u# |8 E: _5 U10 Immunohistochemical Staining for the Detection of the
. v% L, u- D& h) ]Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
: v. h4 y2 C$ Q7 K! kMary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
3 c. M( m+ I' M) W+ g& X0 q3 X) A6 C  n( QChang-Won Lee and David L. Suarez
1 }5 b$ V4 G4 r! [. J* X13 Evaluating the Cell-Mediated Immune Response of Avian
' W) R8 s3 O; e( r, XSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
5 U& i# G5 s  C! h9 ?3 b5 c) _# mDarrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
: \6 D; _+ f* s$ DFollowing Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
( ^) p. S5 J4 F2 C% KIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents


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