施嘉和 台湾中央研究院——HBV相关实验室及人物介绍系列

marine0425030

1 简介
之所以介绍他，是因为他在2010plosone上面的一篇文章系统全面彻底地分析了C蛋白上面入核和出核信号问题
Li, H.C., et al., Nuclear export and import of human hepatitis B virus capsid protein and particles. PLoS Pathog, 2010. 6(10): p. e1001162.

2 实验室链接
http://www.ibms.sinica.edu.tw/big5/pages/pi/index.php?id=58

3 研究介绍
本实验室的研究兴趣以在台湾和全世界都相当重要的肝炎病毒为主。我们过去的研究方向着重于利用组织培养系统来深入探讨B型肝炎病毒 (human hepatitis B virus, HBV) 变异型 (variants) 之功能。我们新的研究重点包括
1. 藉由肝病动物模式探讨引发肝炎肝癌之主要机制；
2. 肝干细胞分化和肝炎病毒之关系；
3. 病毒颗粒之分解，重组，和运输。
这些工作将帮助了解肝炎病毒之致病机转，也将帮助抗病毒药物研发找到新的目标。这些工作将和来自于各领域的专家合作，包括免疫学, 流行病学，结构生物学，和胃肠科。

4 文章
1.Le Pogam S, Chua PK, Newman M, and Shih C: Exposure of RNA Templates and Encapsidation of Spliced Viral RNA are Influenced by the Arginine Rich Domain of Human Hepatitis B Virus Core Antigen (HBcAg 165-173). J. Virol. 2005, 79:1871-1887.
2.Wang RYL, Shen CN, Lin MH, Tosh D and Shih C: Hepatocyte-like Cells Transdifferentiated from Pancreatic Origin can Support Replication of Hepatitis B Virus. J. Virol. 2005, 79:13116-13128.
3.Chua PK, Wang RYL, Lin MH, Masuda T, Suk FM, and Shih C: Reduced Secretion of Virions and HBsAg of an HBV Variant Correlates with the Accumulation of the Small S Envelope Protein in ER/Golgi. J. Virol. 2005, 79:13483-13496.
4.Chua PK, Lin MH and Shih C: Potent Inhibition of Human Hepatitis B Virus Replication by a Host Factor Vps4. Virology 2006, 354:1-6.
5.Newman M, Chua PK, Tang FM, Su PY, Shih C. Testing an Electrostatic Interaction Hypothesis of Hepatitis B Virus Capsid Stability by Using an in vitro Capsid Disassembly/Reassembly System.
J. Virol. 2009, 83: 10616-10626.
6.Chua PK, Tang FM, Huang JY, Suen CS, and Shih C. Testing the Balanced Electrostatic Interaction Hypothesis of Hepatitis B Virus DNA Synthesis by Using an In Vivo Charge Re-balance Approach.
J. Virol. 2010, 84: 2340-2351.
7.Liu S, He J, Shih C, Li K, Dai A, Hong Zhou Z, Zhang J. Structural comparisons of hepatitis B core antigen particles with different C-terminal lengths. Virus Research 2010, 149:241-244.
8.Shih, HH, Shih C, Wang HW, Su CW, Sheen, IJ and Wu JC. Pro-205 of Large Hepatitis Delta antigen and Pro-62 of Major Hepatitis B Surface Antigen influence the Assembly of Different Genotypes of Hepatitis D Virus.J. Gen. Virol. 2010, 91:1004-1012.
9.Wang CH, Davamani F, Sue SC, Lee SC, Wu PL, Tang FM, Shih C, Huang TH, Wu WG. Cell surface heparan sulfates mediated internalization of PWWP/HATH domain of HDGF via macropinocytosis to fine-tune cell signaling process involved in and its effect on fibroblast cell migration.  Biochem. J. 2010, (in press).
10.Li HC, Huang EY, Su PY, Wu SY, Yang CC, Lin YS, Chang WC, and Shih C. Nuclear Export and Import of Human Hepatitis B Virus Capsid Protein and Particles. PLoS Pathogens 2010, 6(10): e1001162.


bigben 发表于 2010-12-5 04:20