禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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9 p9 P' o* w. K2 i' T* ]08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
0 r3 v/ j" Z0 w3 C' k【简    介】：
, x: ^7 `5 g* V/ j4 u   08新书,大家共享    附件PDF下载！

   The aim of this book is to create a resource that includes both basic methods that
, n2 G# J2 o  {8 \are currently used and well established as well as some of the most exciting new
" x0 i' P" v# h7 xmethods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
9 v/ s7 f' ]( L3 gwill be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
0 a- _! l' t& BI would like to thank all the authors for their contributions. I am also thankful to
# P- V7 o$ g1 ]7 D% T: AJohn M. Walker, the series editor, for his help in editing and working with me
6 |) j$ }5 l# {2 j* b% D1 ^+ xthrough this project.
1 B# n2 ~- `: g. r+ A" h% w4 `Erica Spackman
! n1 e  A: m' h5 C4 NAthens, GA, September 2007
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【出版日期】：2008
【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
; w. l9 _% [) [* s! U! \8 uContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
. f! B2 N' L% Y& o' S1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
+ \$ b% M. t1 R' |/ \% o: v& nErica Spackman
# i8 T7 W: c; j  u4 |: q8 a, D/ M$ `2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
9 X3 p. \1 y8 wMary Lea Killian
6 K7 }& o  K- T% Y1 o2 Z6 s3 Avian Infl uenza Virus RNA Extraction from Tissue
$ G- M$ T5 [, Mand Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
; j4 o6 ?7 c/ pby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
9 J! f7 z* v' FSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
8 |7 ^2 f* w4 P5 V6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
2 H0 C; H7 ^6 T& j9 GPeter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
7 W" `' r# ]! s* F: Q# c7 _8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
2 L0 A) X, ~& L" v8 Ivii
9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
5 r& Q$ C; t# \  J) }+ WNeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
0 C% w, t8 Z1 H3 V9 h. x/ NAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
' t" B, ]) Z1 `4 k) ]11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
! `2 e8 p* G% s  g/ O! j9 a. K12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
; e) K! f; J$ x+ o# [; Q6 HChang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
: J: a- {! W4 A. Y  \# Q: a( l- qSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
' {( K/ m& }1 t% h1 @# l( L" x8 r4 o5 h- EDarrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
* U9 ?$ d2 ~  d! I. ]8 T' KDarrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents




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