禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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- F8 `! J' l' e* P08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
! `" Q% x* t5 W; p, ]! r【出 版 社】: Humana Press
【简    介】：
   08新书,大家共享    附件PDF下载！

   The aim of this book is to create a resource that includes both basic methods that
are currently used and well established as well as some of the most exciting new
methods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
* X# O- C5 ^6 M9 d8 \will be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
, v7 d! X. s  l7 B) aI would like to thank all the authors for their contributions. I am also thankful to
0 l) q( O4 H3 ~1 _5 wJohn M. Walker, the series editor, for his help in editing and working with me
through this project.
Erica Spackman
Athens, GA, September 2007

【出版日期】：2008
. B9 O! t: F) P# _: g【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
; W( }9 ?6 X' b' T7 h, R" JErica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
Mary Lea Killian
# s4 U8 C. k4 W  ?# p5 r$ O$ D3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
6 Y! Q- n  G) p' iSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
$ I; \  _. A6 L$ N6 Avian Infl uenza Virus Isolation and Propagation
" N3 R. _- f; k5 \8 P' j9 O$ Rin Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
- i. N9 U* `8 B/ E4 EPeter R. Woolcock
, ^0 Z, i3 X3 d' P6 U7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
1 w* i* m; f' U' Q3 |: ~Mary Lea Killian
0 S3 b% x* \8 `# K2 }8 H8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
( P% Z) z2 ]" d4 h# E& z1 Hof Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
( v: Z9 T; \( C5 ^* VJanice C. Pedersen
vii
% N1 F3 O' b) j8 |4 H! j7 S4 v9 Neuraminidase-Inhibition Assay for the Identifi cation
6 b+ R: }8 g. y, T  Rof Infl uenza A Virus Neuraminidase Subtype or
( O" w/ H4 P' j" R+ ~- RNeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
9 N/ P! V( w7 A" ~Janice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
/ q5 ~% y+ K4 PAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
9 Y9 c' x$ A5 M; n2 i" ZJustin D. Brown and David E. Stallknecht
4 B- w1 `2 E+ s4 }% i12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
1 J: o3 F* r6 j+ @9 U: [) \6 h$ X13 Evaluating the Cell-Mediated Immune Response of Avian
Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
2 A6 M* p7 ]( q* t. d: ~14 Measurement of Avian Cytokines with Real-Time RT-PCR
2 N6 C! @- V8 r' EFollowing Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
7 U( a, V5 M+ q) \. Gviii Contents
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