[Journal Club] Mapping Viral Functional Domains for Genetic Diversity in Plants

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Mapping Viral Functional Domains for Genetic Diversity in Plants


Pita JS, Roossinck MJ. 2013. Mapping Viral Functional Domains for Genetic Diversity in Plants. J. Virol. 87:790–797.
粗略的中文摘要。。。
文章通过比较FNY 和LS两个株系的分离物寻找在植物里面控制遗传变化的因子。探究发现RNA1, RNA2控制遗传多样性的水平。进一步研究发现突变频率的控制因子在RNA1的前596氨基酸位置。2A蛋白中与2A/2B的重叠区域也与病毒遗传变化有关。在含有大多数固定突变的RNA3的3UTR发现一个多态性热点。沉默抑制因子2B基因通过依赖于寄主控制病毒种群的固定突变丰度

摘要：
Cucumber mosaic virus (CMV) comprises numerous isolates with various levels of in-host diversity. Subgroup-distinctive features of the Fny and LS strains provided us with a platform to genetically map the viral control elements for genetic variation in planta. We found that both RNAs 1 and 2 controlled levels of genetic diversity, and further fine mapping revealed that the control elements of mutation frequency reside within the first 596 amino acids (aa) of RNA 1. The 2a/2b overlapping region of the 2a protein also contributed to control of viral genetic variation. Furthermore, the 3′ nontranslated region (NTR) of RNA 3 constituted a hot spot of polymorphism, where the majority of fixed mutations found in the population were clustered. The 2b gene of CMV, a viral suppressor of gene silencing, controls the abundance of the fixed mutants in the viral population via a host-dependent mechanism
链接：
http://jvi.asm.org/content/87/2/790.full