Beet necrotic yellow vein virus

donggua

J Gen Virol January 2012 vir.0.039685-0

Beet necrotic yellow vein virus subgenomic RNA3 is a cleavage product leading to stable non-coding RNA required for long distance movement
Claire Peltier , Elodie Klein , Kamal Hleibieh , Massimiliano D'Alonzo , Philippe Hammann , Salah Bouzoubaa , Claudio Ratti  and David Gilmer

Beet necrotic yellow vein virus is a multipartite RNA virus. BNYVV RNA3 does not accumulate in non-host transgenic Arabidopsis thaliana plants when expressed using a 35S promoter. However a 3' derivative species has been detected in transgenic plants and in transient expression assays conducted in Nicotiana benthamiana and Beta macrocarpa. The 3' derivative species is similar to the previously reported subgenomic RNA 3 produced during viral infection. 5' RACE revealed that the truncated forms had identical 5' ends. The 5' termini carried the coremin motif also present on BNYVV RNA5, Beet soil borne mosaic virus RNA3 and 4 as well as Cucumber mosaic virus group 2 RNAs. This RNA3 species lacks a m7Gppp at the 5' end of the cleavage products, whether transiently or virally expressed. Mutagenesis revealed the importance of the coremin sequence for both long distance movement and stabilization of the cleavage product in vivo and in vitro. The isolation of various RNA3 5' end products suggests the existence of a cleavage between nts212 and 1234 and subsequent exonucleolytic degradation leading to the accumulation of a non-coding RNA. When RNA3 was incubated in wheat germ extracts, truncated forms appeared rapidly and their appearance was protein and divalent ion dependant.

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http://vir.sgmjournals.org/conte ... 0.039685-0.abstract