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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
' ?  g8 U' H. {$ R  y4 V' _$ PYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
6 V- Q. O- s6 F5 g  tTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
6 x' X, h+ G8 ~5 ?6 Lkey role in controlling viral infections. In this study, we report the cloning, expression, and
% T0 p1 E# k3 d7 J9 ebiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
- w, O8 D$ _6 k0 Z! H" l% Ademonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
2 D: X/ u4 Y/ ~* QM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
2 J, X9 ^1 \4 u0 x! V! P( p8 u6 wIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
- A- u6 a5 Z% o. x2 x( `' i3 |species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
/ T7 u! B6 A+ a3 I+ S" X9 \activity in microbats, which has important implications for virus interactions with these hosts.
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& x3 P' V- m6 ]+ c2 Y4 v% s6 UKeywords: bat; interferon; IFN-stimulated genes; antiviral activity
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# a3 m" p2 E& I' I2 a中文信息：
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( `) @' Q1 y9 f" m0 l9 u大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测


* l) d0 |( f& K2 A梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*


, c  F/ F2 u! e! _9 w摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。

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" G7 v6 c( v* P+ d关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR

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