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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*

4 g# L! K. n3 o7 pAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
: q( s/ U% N. |% p. L7 Q9 |( N+ TTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
' D: \+ g$ M" m9 yinformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
  m1 q  C5 X+ Z) L1 Z  [key role in controlling viral infections. In this study, we report the cloning, expression, and
, D4 m4 O, w5 t6 U7 ~( H- O! U  I! qbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
" @2 \) X& l0 d, u9 lM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
' ]# U; f7 j5 z& O# A! u; Cspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
activity in microbats, which has important implications for virus interactions with these hosts.
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( w: t% H, [) C, x8 f. NKeywords: bat; interferon; IFN-stimulated genes; antiviral activity

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- q  E/ o- U& E+ v9 V0 E中文信息：
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5 v( r" B5 C3 ?大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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2 @8 n4 ~! V9 S8 Z* z2 ]梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*


摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。


关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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