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【书名版次】: Adeno-Associated Virus: Methods and Protocols (Methods in Molecular Biology)
( y4 B h8 g6 i ~& l5 U【作 者】:Richard O. Snyder (Editor), Philippe Moullier (Editor)- [) O4 S! Z7 C' w; Z
【出 版 社】: Humana Press; 2011 edition
8 h& G p6 r, M; z【出版日期】: (November 30, 2011) }% ^7 w" I* V# Q& z( a! `
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【简 介】: Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.8 G5 }+ s. ^( b
【目 录】:
% ?: c! f6 S3 Y( jHardcover: 475 pages$ x" g: I" G; q. a+ f
Language: English
" X9 Q- q; t7 mISBN-10: 1617793698
% M- w8 U8 j( gISBN-13: 978-1617793691
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