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发表于 2015-2-3 19:20:34
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" f. E3 U9 l7 a7 iIMMUNOLOGY
; c0 E- w( ~/ D7 x# ~' Z60.401' I; R: D# E+ T) \+ y( S6 p# F
LAB MANUAL 2004
2 j9 T+ D7 g3 m3 E, }0 ~不错的书,下面是目录 8 T% H) q/ c9 J, S% `7 x
SCHEDULE 2/ u) C* E, D; P: _
GENERAL INSTRUCTIONS 4 6 s# o# s. X- y3 R6 C% U) M/ O
REFERENCE BINDER CONTENTS 8
- z* { P) f/ m! d4 iSTANDARD OPERATIONS PROCEDURE 9
/ R4 _3 \; i2 @# V+ DWHMIS 11# f) c% |' ?( I! o8 ^% ~
Lab 1: Preparation of Crude IgG by Salt Fractionation 13! `6 ]4 ?4 {' K
Lab 2: Immunoprecipitation 17
* [- o( b* F/ YLab 3: Hemagglutination Assay 28, n% A( j; j2 Z$ H3 h0 H9 `6 b* u( y
Lab 4: Introduction to the Enzyme Linked Immunosorbent Assay (ELISA) 33
$ [( C. [ u {! S8 QLab 5: Part I: Induction and characterization of a primary cytokine 38# c/ l) L! A! K' M
response in vivo.+ U _( S( L7 I+ e0 A, b+ U
Part II: ELISA precision training.- p- u Y, |% L& y( q4 T1 O
Lab 5: Part III: ELISA ASSAY- Quantitative analysis of IL-13 Response 476 E( x8 n7 x, H6 C; W
Appendix:
/ K @4 P- S/ e" M, aSolutions 53
( `" k. q3 \; e5 AFloor Model Centrifugation Operation 540 y1 ?2 _, `# g7 {0 E2 b0 M# n) Z
Pipetman Operation 56: e3 O* A+ W/ n1 K5 w; B
Electrophoresis 58* m6 _& T8 p( B2 v3 l
Sample Lab Exam 60 |
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