【转移帖】HBV哪些基因整合到基因组上呢?

cao1976

原帖由davay'发表于 2009-7-24 23:47 ：

HBV好象可以发现，可以整合到肝细胞的基因组上,但是我一直不清楚，是整个HBV基因组呢?还是个别基因整合呢?

cao1976

xray19发表于 2009-7-25 00:36 ：

记得好像大多是部分片段整合，以X基因居多。  

cao1976

bigben发表于 2009-7-25 02:28 ：
片段居多吧，有HBs，整个的或者多拷贝串联基因组不太清楚
看图λIA22好像超过1copy

看下面的图，来自
Nagaya, T., et al. , The mode of hepatitis B virus DNA integration in chromosomes of human hepatocellular carcinoma. Genes & development, 1987. 1(8): p. 773-782.

解说：
Figure  1.  Structure  of the  integrated HBV sequences.  At  the  top of the  figure,  the whole HBV genome  is  represented.  The  long  thin line  shows  the minus strand with  the DNA-linked protein  (0),  the  short  thin  line  represents  the plus  strand. The  length  of the HBV genome  is  indicated  in kilobases,  numbering  from  the hypothetical  EcoRI  site of sybtype adr (Ono et  al.  1983; Fujiyama et  al.  1983).
Open reading frames and their direction of transcription  are represented by filled arrows. (S) HBsAg (preS), pre-S region;  (C) HBcAg;  (X) X gene. The P gene is omitted  for clarity. DR1, DR2 are the  11-bp direct repeats whose starting points are 1590 and  1824, respectively. The  region  in between DR1  and DR2  is  the  cohesive  end  region.  HBV genome  regions  that  are covered by  the  integrated  virus DNA are shown by solid bars. The virus DNA  that has one continuous  portion(s)  of a circular HBV genome  is represented by a single  solid bar aligned  in  the  same level. Deletions  in  the genome  are represented by dotted  lines. The  complex-type virus DNA that  consists  of two  or more HBV genomes whose  regions  are overlapping,  at  least  in part,  is  represented  by multiple  solid  bars  aligned  in  different levels,  each representing  different units  of the virus genome,  and connected  by tilted  dotted  lines.  Solid  thin  lines  represent flanking human DNA. Encircled numerals  show  the number of the human  chromosome  in which  the flanking DNA was assigned (see text). L indicates  that  the cloning vector is directly joined  to the virus DNA. Alu  and Satellite III indicate  that  the cell DNA at the junction  is homologous  to  either Alu  repeating  sequence  or Satellite  III sequence,  respectively.

cao1976

bigben发表于 2009-7-25 02:35

Distribution of the viral integration sites on the chromosome map
Target genes searched by viral insertion

来自
Murakami, Y., et al. , Large scaled analysis of hepatitis B virus (HBV) DNA integration in HBV related hepatocellular carcinomas. Gut, 2005. 54(8): p. 1162-8.

cao1976

davay发表于 2009-7-25 13:01 ：正是我要的，非常感谢。

cao1976

davay发表于 2009-7-25 13:01 ：正是我要的，非常感谢。

cao1976

emma0802发表于 2010-6-20 20:21 ：

看了几个帖子，对各位版主的敬仰犹如…………也不知道各位是看了超多的文献还是具有超强的整理知识的能力