[转移帖]中国农大：猪乙肝病毒可能存在

cao1976

原帖由bigben发表于 2010-3-31 20:27

Prevalence of a virus similar to human hepatitis B virus in swine
Author: Li, W.; She, R.; Liu, L. (...)
Source: Virol J, 2010, 7(1): 60
http://www.virologyj.com/content/7/1/60

没来得及看全文。有意思的文章。
该篇文章由中国农大发表，声称在猪内发现嗜肝DNA病毒的亚种，其HBsAg检出率在猪内约为1/4。有Elisa，免疫组化，电镜结果，不知道为什么没有把基因组给弄出来。

Background
The objective of this study is to established evidence of the existence of a novel member of the hepadnavirus family endemic in swine. Temporarily this virus was designated as swine hepatitis B virus (SHBV). This SHBV can be detected by using human hepatitis B virus diagnostic kits including ELISA, immunohistochemical staining, and transmission electron microscopy (TEM). Also seroprevalence of pig farms in Beijing, China, and pathological features of SHBV infection was determined.

Results
Screened result shows that overall prevalence of HBsAg was 24.8%, closed to that of anti-HBsAg, whereas HBeAg and anti-HBe were barely detectable. The distribution of HBsAg and HBcAg was examined by immunohistochemistry of liver samples. Typical hepatitis pathological change, such as spotty parenchymal cell degeneration, necrosis of hepatocytes and proliferation of fibrous connective tissue were observed during histopathological analysis. Analysis of HBsAg-positive serum with TEM revealed two morphologic forms, 20nm and 40nm sized particles, similar to small spherical and Danes particles of HBV. Observation of the ultrastructure of the liver also found HBV-like particles in the nucleus of hepatocytes.

Conclusion
Our research result implies that SHBV could be a causative agent of swine. The discovery of SHBV will unveil novel evolutionary aspects of hepatitis and provides new information for further hepadnavirus research.

cao1976

dudiaojiangxue 发表于 2010-4-1 09:00 ：
这就好玩儿了，乙肝研究正缺动物模型呢。现有的DHBV鸭肝模型由于和HBV差别比较大，一直不太理想。所以很多研究组一直在做老鼠的动物模型，但老鼠不能直接被HBV感染的，所以还得做嵌合体，总之麻烦大了。猪动物模型有可能会缓解乙肝动物模型缺乏这一问题。

正如斑竹所说该研究没有放DNA结果，我的想法是农大把两个结果拆开了，DNA结果绝对能发一篇JV，期待这个结果能早点儿面市

太可惜了，才发了1.8的影响因子。他这篇工作非常重要，与他的实验内容少无关。如果我是作者我会发到JV或者PNAS或者Hepatology等杂志，以letter或者report的形式发表，太可惜了，不知道自己做得工作多么重要！我搜了一下，97年在swine上发现了sHEV都发表在了PNAS上。

cao1976

davay发表于 2010-4-1 18:27 ：

我觉得没有genome的结果，就不能确定这个是HBV病毒的，HBsAg阳性可能是由于kit的原因  

个人估计该文 序列应该是99%没有拿到的

cao1976

yaoming发表于 2010-4-3 22:41 ：

科学研究讲究一个实实在在的东西。

我估计是他们做不出来，要拿到动物体内的HBV DNA，cccDNA结果谈何容易。一定要用核酸分子杂交技术做出来才算！

他们应该要拿到这个序列嘛！不知道怎么这么急于发文章。不会连全基因组都克隆不出来吧！