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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)7 E' D2 j" j M
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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" g- T l1 o/ U) `1 c1 uAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.# W2 [' c y3 X; } |
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little8 e+ c# o, G4 o" C" V
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
+ a& {# c, W1 t' B" L& @key role in controlling viral infections. In this study, we report the cloning, expression, and
, z- _* d& |& Y9 d$ \- ]biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We; ]7 h7 N" Q9 a+ z
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
7 b7 N2 m2 |' z: ~6 I$ a# K+ Y# HM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
f) ]/ v0 G$ y, z/ K4 EIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
% F7 i- S" L4 S. O) z5 g$ ~, N6 Bspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral: j/ N& }/ b/ z- r- }# Y$ [
activity in microbats, which has important implications for virus interactions with these hosts.
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3 D8 z; n* S9 WKeywords: bat; interferon; IFN-stimulated genes; antiviral activity
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# r& G) S9 a, ?( [+ Q% C3 y( R中文信息:
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& n2 K9 E: y& y$ g大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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8 g* R5 U" {' W梁英子,吴利军,张倩,周鹏,王媚娘,杨兴娄,葛行义,王林发,石正丽*
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; O) l* Q4 z; @4 t. \摘要:蝙蝠是多种病毒的自然宿主,其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状,其中机制未知。近期对两种蝙蝠基因组序列分析结果提示,蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上,用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平;表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白,测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用,试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。* R, }0 P" }% H4 w% v0 Z
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, W3 G# U0 o0 g7 [/ }& w8 C$ R: u关键词:干扰素,蝙蝠天然免疫,抗病毒活性,定量PCR/ @; ~. n+ {$ l6 h+ Y3 H
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