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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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& w+ @5 [: d) `- Y# B+ ~8 dAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
, q2 t7 I0 b, d! A0 WTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
; @6 e7 V. }2 K) J- ukey role in controlling viral infections. In this study, we report the cloning, expression, and
, y/ a# E/ c/ ^# p  rbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
4 f6 \! M% }5 t& f6 D4 Pdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
- q5 p: U5 J# R% [' |$ p" e9 Z/ MM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
  |$ ]3 c' M8 \; D1 ~  [species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
activity in microbats, which has important implications for virus interactions with these hosts.
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3 O3 ^- I5 c7 E; ~% [$ s7 t+ F1 hKeywords: bat; interferon; IFN-stimulated genes; antiviral activity
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8 d5 R: d- X% x: _3 l* w! ~中文信息：

. J  n( Q' c0 f: c大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*

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( p! X/ r) O0 u- u3 Y- H/ Y摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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! x) L/ w6 c- _7 _. E# a关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR

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