|
Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)8 _9 E- C9 F3 t7 W
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
% K7 L4 g3 M+ t0 _: }! N
# y# t% A$ s3 s5 m$ X) T3 D# GAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
* _- [/ \, t% \5 dTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
( B0 l: l4 u( d Zinformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a+ O+ K9 f& L+ k! D9 s
key role in controlling viral infections. In this study, we report the cloning, expression, and
; t9 n8 j {/ S4 r, K& Qbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
& p. z" ^, W; D, a- J% d6 O5 r Pdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from3 s& u- h9 h7 f+ |3 v( ?2 C/ c( E* a
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant6 m; _; a n& a* ?1 M* v8 |0 \. h l
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat! u: d, Q: M/ U: ~4 t( R
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral. P; b( m8 x5 ~8 U" }' w% l/ ]
activity in microbats, which has important implications for virus interactions with these hosts.
; b) v2 m: \' L* X% ?2 ]' F% T2 h- ~! c1 t8 X5 P
Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
0 i+ f* ^, ]* C& {% c7 |3 a6 j2 z" M' }$ H+ t/ i3 J
% J& q$ h3 ^3 I' Y s, I( H3 L
中文信息:. {- z: b* W9 I( M
7 ?5 O, p" n5 k6 J大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测8 U) n$ L3 m5 w7 f0 g
7 }: n" b6 _: `4 U2 V" j5 n$ Q4 d+ K F7 w4 b* C" g) Q
梁英子,吴利军,张倩,周鹏,王媚娘,杨兴娄,葛行义,王林发,石正丽*$ Z- |( Q% e! L0 Q2 Q
& V% [5 B0 N% _3 g6 B6 I9 l) [. c9 g {" n: j* Z# h$ y
摘要:蝙蝠是多种病毒的自然宿主,其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状,其中机制未知。近期对两种蝙蝠基因组序列分析结果提示,蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上,用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平;表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白,测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用,试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
( ~0 e: S2 t2 [1 k7 y% ]/ S A7 b
# O3 K2 e: d; f" {& k3 \! P) b
% F# w! G- K v4 R6 m关键词:干扰素,蝙蝠天然免疫,抗病毒活性,定量PCR
3 o* `- ]* W1 @ E' U8 w. d- s0 a2 x7 ]* ^3 K
|
|