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【书名版次】: Adeno-Associated Virus: Methods and Protocols (Methods in Molecular Biology) P: c! U1 L6 n. z0 T5 f8 b
【作 者】:Richard O. Snyder (Editor), Philippe Moullier (Editor)% m: V4 j. R7 z2 @
【出 版 社】: Humana Press; 2011 edition
6 y- d# M% ~& _- U; }【出版日期】: (November 30, 2011)
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【简 介】: Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.* M1 m3 [4 S# K+ i2 k. }
【目 录】:
1 f. ^. h4 N5 F+ R) iHardcover: 475 pages# T7 O1 N' g/ n5 M9 Y
Language: English: J, l8 r% _2 c
ISBN-10: 1617793698! }' \4 x; N/ `6 V" ~$ |
ISBN-13: 978-1617793691: U1 m& B0 i, \4 `/ ]0 _! i5 F
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