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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)
9 m U( i" _% u0 Z& T lYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.) s' L: g9 B4 |3 E, r3 t
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little$ G3 l' q5 L- W
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
+ F' s2 {# K$ `0 c" k3 o& J0 Dkey role in controlling viral infections. In this study, we report the cloning, expression, and
% d3 x. N* e; Wbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
% p3 z) X. i* Udemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from( ?. t/ ^( ]7 v, C( K
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant d) {$ a5 P$ ~
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
' B* J/ w) C# N+ _) G3 n7 [8 ospecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral% j& C; b, ?7 E/ Y( g8 D
activity in microbats, which has important implications for virus interactions with these hosts./ i+ H& [- j3 N. m
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
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中文信息:
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$ M; F2 Z: \6 ^+ n( P5 C大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测 K8 @4 q" b! I' K( s- H
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$ a4 e: ?9 H' f+ I. m$ p/ d. j% d梁英子,吴利军,张倩,周鹏,王媚娘,杨兴娄,葛行义,王林发,石正丽*
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, v/ J1 h o- X/ H1 F* Z摘要:蝙蝠是多种病毒的自然宿主,其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状,其中机制未知。近期对两种蝙蝠基因组序列分析结果提示,蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上,用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平;表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白,测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用,试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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9 g" d; _- K6 e. K& v关键词:干扰素,蝙蝠天然免疫,抗病毒活性,定量PCR) u% N1 M: b. S" V( \" z
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