The Journal of Molecular Diagnostics: March 2019 (Volume 21, Issue2)

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	Review
	Molecular Pathology of Bone Tumors Suk Wai Lam, David G.P. van IJzendoorn, Anne-Marie Cleton-Jansen, Karoly Szuhai, Judith V.M.G. Bovée
	Commentary
	Targeted RNA Sequencing in Non–Small Cell Lung Cancer Lauren L. Ritterhouse
	Technical Advances
	A Novel Approach to Detect Programed Death Ligand 1 (PD-L1) Status and Multiple Tumor Mutations Using a Single Non–Small-Cell Lung Cancer (NSCLC) Bronchoscopy Specimen Amanda Vannitamby, Shona Hendry, Tanvi Makadia, Janine Danks, John Slavin, Louis Irving, Daniel Steinfort, Steven Bozinovski Open Access
	Combined Targeted Resequencing of Cytosine DNA Methylation and Mutations of DNA Repair Genes with Potential Use for Poly(ADP-Ribose) Polymerase 1 Inhibitor Sensitivity Testing Christina Grimm, Axel Fischer, Angela M. Farrelly, Roshni Kalachand, Roberta Castiglione, Elena Wasserburger, Michelle Hussong, Anne M. Schultheis, Janine Altmüller, Holger Thiele, H. Christian Reinhardt, Kai Hauschulz, Bryan T. Hennessy, Ralf Herwig, Matthias Lienhard, Reinhard Buettner, Michal R. Schweiger
	Regular Articles
	A Novel Multiplex Droplet Digital PCR Assay to Identify and Quantify KRAS Mutations in Clinical Specimens Miguel Alcaide, Matthew Cheung, Kevin Bushell, Sarah E. Arthur, Hui-Li Wong, Joanna Karasinska, Daniel Renouf, David F. Schaeffer, Suzan McNamara, Mathilde Couetoux du Tertre, Gerald Batist, Hagen F. Kennecke, Aly Karsan, Ryan D. Morin
	Clinical Utility of a Next-Generation Sequencing Panel for Acute Myeloid Leukemia Diagnostics Carmen M. Alonso, Marta Llop, Claudia Sargas, Laia Pedrola, Joaquín Panadero, David Hervás, José Cervera, Esperanza Such, Mariam Ibáñez, Rosa Ayala, Joaquín Martínez-López, Esther Onecha, Inmaculada de Juan, Sarai Palanca, David Martínez-Cuadrón, Rebeca Rodríguez-Veiga, Blanca Boluda, Pau Montesinos, Guillermo Sanz, Miguel A. Sanz, Eva Barragán
	Mutation Burden and I Index for Detection of Microsatellite Instability in Colorectal Cancer by Targeted Next-Generation Sequencing Jeong E. Kim, Sung-Min Chun, Yong S. Hong, Kyu-pyo Kim, Sun Y. Kim, Jihun Kim, Chang Ohk Sung, Eun J. Cho, Tae W. Kim, Se Jin Jang
	Optimizing a Metatranscriptomic Next-Generation Sequencing Protocol for Bronchoalveolar Lavage Diagnostics Weihua Huang, Changhong Yin, Guiqing Wang, Jeremy Rosenblum, Sankaran Krishnan, Nevenka Dimitrova, John T. Fallon
	Somatic Tumor Variant Filtration Strategies to Optimize Tumor-Only Molecular Profiling Using Targeted Next-Generation Sequencing Panels Mahadeo A. Sukhai, Maksym Misyura, Mariam Thomas, Swati Garg, Tong Zhang, Natalie Stickle, Carl Virtanen, Philippe L. Bedard, Lillian L. Siu, Tina Smets, Gert Thijs, Steven Van Vooren, Suzanne Kamel-Reid, Tracy L. Stockley
	Development of Novel Mutation-Specific Droplet Digital PCR Assays Detecting TERT Promoter Mutations in Tumor and Plasma Samples Broderick C. Corless, Gregory A. Chang, Samantha Cooper, Mahrukh M. Syeda, Yongzhao Shao, Iman Osman, George Karlin-Neumann, David Polsky
	Rapid and Sensitive Detection of Azole-Resistant Aspergillus fumigatus by Tandem Repeat Loop-Mediated Isothermal Amplification Ling-Shan Yu, Jesus Rodriguez-Manzano, Kenny Malpartida-Cardenas, Thomas Sewell, Oliver Bader, Darius Armstrong-James, Matthew C. Fisher, Pantelis Georgiou
	Software-Assisted Manual Review of Clinical Next-Generation Sequencing Data: An Alternative to Routine Sanger Sequencing Confirmation with Equivalent Results in >15,000 Germline DNA Screens Dale Muzzey, Shera Kash, Jillian I. Johnson, Laura M. Melroy, Piotr Kaleta, Kelly A. Pierce, Kaylene Ready, Hyunseok P. Kang, Kevin R. Haas Open Access
	Next-Generation Sequencing–Based Assessment of JAK2, PD-L1, and PD-L2 Copy Number Alterations at 9p24.1 in Breast Cancer: Potential Implications for Clinical Management Sounak Gupta, Chad M. Vanderbilt, Paolo Cotzia, Javier A. Arias-Stella III, Jason C. Chang, Ahmet Zehir, Ryma Benayed, Khedouja Nafa, Pedram Razavi, David M. Hyman, José Baselga, Michael F. Berger, Marc Ladanyi, Maria E. Arcila, Dara S. Ross
	A Rigorous Interlaboratory Examination of the Need to Confirm Next-Generation Sequencing–Detected Variants with an Orthogonal Method in Clinical Genetic Testing Stephen E. Lincoln, Rebecca Truty, Chiao-Feng Lin, Justin M. Zook, Joshua Paul, Vincent H. Ramey, Marc Salit, Heidi L. Rehm, Robert L. Nussbaum, Matthew S. Lebo Open Access
	Establishment of Immunoglobulin Heavy (IGH) Chain Clonality Testing by Next-Generation Sequencing for Routine Characterization of B-Cell and Plasma Cell Neoplasms Maria E. Arcila, Wayne Yu, Mustafa Syed, Hannah Kim, Lidia Maciag, JinJuan Yao, Caleb Ho, Kseniya Petrova, Christine Moung, Paulo Salazar, Ivelise Rijo, Tessara Baldi, Ahmet Zehir, Ola Landgren, Jae Park, Mikhail Roshal, Ahmet Dogan, Khedoudja Nafa
	t(3;8)(q26.2;q24) Often Leads to MECOM/MYC Rearrangement and Is Commonly Associated with Therapy-Related Myeloid Neoplasms and/or Disease Progression Guilin Tang, Shimin Hu, Sa A. Wang, Wei Xie, Pei Lin, Jie Xu, Gokce Toruner, Ming Zhao, Jun Gu, Madison Doty, Shaoying Li, L. Jeffrey Medeiros, Zhenya Tang
	Design, Optimization, and Multisite Evaluation of a Targeted Next-Generation Sequencing Assay System for Chimeric RNAs from Gene Fusions and Exon-Skipping Events in Non–Small Cell Lung Cancer Richard A. Blidner, Brian C. Haynes, Stephen Hyter, Sarah Schmitt, Ziyan Y. Pessetto, Andrew K. Godwin, Dan Su, Patrick Hurban, Léon C. van Kempen, Maria L. Aguirre, Shobha Gokul, Robyn D. Cardwell, Gary J. Latham