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[细菌] 大肠杆菌致病岛重定向助手噬菌体包装

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发表于 2019-7-26 11:14:10 | 显示全部楼层 |阅读模式
Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit

Highlights

PICI Rpp protein promotes PICI transfer while blocking phage reproduction

Rpp forms a heterocomplex with helper phage TerS

Crystal structures of Rpp alone or complexed with TerS were determined

TerS complexed with Rpp switches specificity from the phage DNA to the PICI genome
Summary
Phage-inducible chromosomal islands (PICIs) represent a novel and universal class of mobile genetic elements, which have broad impact on bacterial virulence. In spite of their relevance, how the Gram-negative PICIs hijack the phage machinery for their own specific packaging and how they block phage reproduction remains to be determined. Using genetic and structural analyses, we solve the mystery here by showing that the Gram-negative PICIs encode a protein that simultaneously performs these processes. This protein, which we have named Rpp (for redirecting phage packaging), interacts with the phage terminase small subunit, forming a heterocomplex. This complex is unable to recognize the phage DNA, blocking phage packaging, but specifically binds to the PICI genome, promoting PICI packaging. Our studies reveal the mechanism of action that allows PICI dissemination in nature, introducing a new paradigm in the understanding of the biology of pathogenicity islands and therefore of bacterial pathogen evolution.


https://doi.org/10.1016/j.molcel.2019.06.017
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