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[真菌] Fungi accelerate pancreatic cancer

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发表于 2019-10-18 16:03:57 | 显示全部楼层 |阅读模式
The impact of fungi on human health is under-studied and underappreciated. One genus of fungus, Malassezia, has now been linked to the progression of pancreatic cancer.

Ivy M. Dambuza &
Gordon D. Brown






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The communities of microorganisms that occupy specific regions of the body are often altered in cancer1, and these microbiomes — particularly their bacterial components — are a current focus of cancer research. One example is pancreatic ductal adenocarcinoma (PDA), for which changes in the bacterial community occupying the pancreas have been documented2. This lethal disease often goes undetected until it has reached advanced stages, and the prognosis is usually very poor3. Writing in Nature, Aykut et al.4 reveal that the fungal component of the pancreatic microbiome (known as the mycobiome) is also altered in PDA. In fact, an abundance of a specific fungal genus actually promotes the disease.

Read the paper: The fungal mycobiome promotes pancreatic oncogenesis via activation of MBL
The mycobiome is a historically under-recognized player in human health and disease, but its role in both is essential. Harmless organisms called commensals, including fungi, inhabit mucosal surfaces such as the linings of the gut, nose and mouth, and can activate inflammatory processes as part of the immune system’s response to injury or infection. In some cases, changes in the biodiversity of fungal communities are linked to aggravated inflammatory-disease outcomes. For example, intestinal overgrowth of Candida albicans— a fungus that causes oral thrush in babies — has been associated with severe forms of intestinal ulcers5 and with mould-induced asthma6. Moreover, it is becoming apparent that there is a relationship between the gut mycobiome and human cancers, including colorectal and oesophageal cancer7.
Aykut et al. used DNA sequencing to search for fungus-specific genomic markers in the cancerous pancreas. This revealed increased pancreatic fungal colonization, both in humans who have PDA and in experimental mouse models of PDA, compared with the pancreas of healthy counterparts. What is the source of these fungi? The authors introduced a fluorescently tagged fungal strain into the guts of mice, and the fungus could be detected in the pancreas as early as 30 minutes later. It is known that there is a direct link between the gut and the pancreatic duct, and microbial translocation into the pancreas has been seen for other organisms8, but not previously for fungi.
The researchers then investigated the link between pancreatic tumour development and fungi using mice engineered to express a cancer-causing protein in the pancreas. These mice develop a slowly progressive PDA that recapitulates the human disease. The mycobiome of the pancreas was notably different from that of the gut in the mutant mice, although the mechanisms underlying this difference are unclear. One genus of yeast, Malassezia, was much more prevalent in pancreatic tumours than in either the guts of these animals or the pancreas of healthy animals. Importantly, Malassezia was also prevalent in human PDA samples.

Weighing in on weight loss in pancreatic cancer
Malassezia species have been best studied in skin conditions such as dandruff and atopic dermatitis. Indeed, they are the most abundant fungal species in mammalian skin, accounting for more than 80–90% of the skin’s commensal mycobiome9. Because we are constantly exposed to Malassezia, healthy individuals can have immune responses to the genus, which in some cases lead to disease. For instance, inflammation caused by overgrowth of Malassezia can worsen gastric ulcers10.
This information hinted that the abundance of Malassezia in PDA tumours could be medically relevant. Indeed, Aykut et al. found that antifungal drugs halted PDA progression in mice, and improved the ability of chemotherapy to shrink the tumour. Subsequent repopulation of the antifungal-treated animals with a Malassezia species accelerated PDA growth again.
Next, Aykut and colleagues asked how Malassezia promotes PDA growth. Gene-expression analysis revealed that poor survival outcome in human PDA was associated with expression of a molecule called mannose binding lectin (MBL).
MBL is a soluble protein produced in the liver that binds carbohydrates on the surface of microorganisms and then activates a protein system called the complement cascade in the blood. The complement cascade serves a variety of immune functions, including activating immune cells to ingest and kill fungi and other pathogens. The cascade has also been linked to tumour development, because its pro-inflammatory pathways stimulate the growth, survival and motility of cells — including cancer cells. In a final set of experiments, Aykut et al. found that PDA progression was delayed in mice lacking MBL or a key component of the complement cascade called C3, even if Malassezia was present in the pancreas. Thus, Malassezia augments PDA progression by promoting pancreatic inflammation through the complement cascade (Fig. 1).
Figure 1 | Fungi called Malassezia promote pancreatic ductal adenocarcinoma. Aykut et al.4 report that the community of fungi that inhabits the pancreas is altered when mice or humans have the cancer pancreatic ductal adenocarcinoma (PDA), with species of the genus Malassezia becoming particularly abundant. The extracellular protein mannose binding lectin (MBL) recognizes an unidentified carbohydrate structure expressed by Malassezia and activates the protein C3, triggering an inflammatory immune response called the complement cascade. Complement activation has many effects, including stimulation of cell growth, survival and migration — factors that fuel tumour growth.

Aykut and colleagues’ results reveal a previously unappreciated role for fungi in PDA progression. A valuable next step will be to determine whether this role somehow involves interactions with the bacterial species known to promote PDA progression3. Fungi and bacteria coexist in the gut and other mucosal sites, and it is likely that alterations in one community will affect the other. In some scenarios, disease-specific coexistence of bacteria and fungi has been noted — for instance, bacteria of the genus Pseudomonas are often isolated from the lungs of people with cystic fibrosis, which are often infected with fungi called Aspergillus10. Understanding these microbial networks will further enhance our understanding of disease progression and inform therapeutic interventions.
Another unresolved question is how MBL and the complement system integrate with the rest of the immune system during PDA progression. For example, how do MBL and the complement cascade interact with the signalling pathways triggered by an immune-cell receptor protein called dectin-1? This protein recognizes the fungal cell wall and activates protective antifungal immune pathways, often in collaboration with other receptors, including those that recognize the complement cascade. In addition, dectin-1 can directly recognize proteins on tumour cells and modulate the activity of tumour-killing immune cells11. But dectin-1 can also associate with tumour-recognizing receptors, which can promote PDA progression12. Thus, it is clear that we need a much better understanding of the complex interplay between the components of the immune system that target fungi and those that target tumours.
This study highlights a role for fungi in the development of cancer. Excitingly, the work points to the possibility of new therapeutic approaches. Perhaps altering microbial communities by directly targeting specific populations could help ameliorate PDA. Alternatively, therapies targeting immune components such as MBL that control fungal infections could provide a route to combat this lethal cancer.

Nature 574, 184-185 (2019)


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